Cers5^tm1.1Mhoc
Targeted Allele Detail

Summary

• Symbol: Cers5^tm1.1Mhoc
• Name: ceramide synthase 5; targeted mutation 1.1, Michael Hoch
• MGI ID: MGI:6121369
• Synonyms: Cers5^lacZ
• Gene: Cers5 Location: Chr15:99633473-99670396 bp, - strand Genetic Position: Chr15, 56.13 cM
• Alliance: Cers5^tm1.1Mhoc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:232428
• Parent Cell Line: HM-1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd-Hprt1^b-m3

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A NLS-lacZ gene and a FRT-flanked neomycin resistance gene replaced the complete coding sequence via homologous recombination. Flp-medidated recombination removed the neomycin resistance gene. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. (J:232428)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cers5 Mutation: 35 strains or lines available

References

• Original: J:232428 Gosejacob D, et al., Ceramide Synthase 5 Is Essential to Maintain C16:0-Ceramide Pools and Contributes to the Development of Diet-induced Obesity. J Biol Chem. 2016 Mar 25;291(13):6989-7003
• All: 1 reference(s)