Dstyk^tm1.2Zlh
Targeted Allele Detail

Summary

• Symbol: Dstyk^tm1.2Zlh
• Name: dual serine/threonine and tyrosine protein kinase; targeted mutation 1.2, Ze-Lan Hu
• MGI ID: MGI:6121070
• Gene: Dstyk Location: Chr1:132345293-132394696 bp, + strand Genetic Position: Chr1, 57.24 cM, cytoband E4
• Alliance: Dstyk^tm1.2Zlh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:226871
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: A loxP site was inserted into intron 3 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 13. The neo cassette was removed through subsequent flp-mediated recombination. Exons 4-13 were deleted through subsequent cre-mediated recombination. Exons 8-13 code for the kinase domain. qRT-PCR and western blots confirmed the absence of expression from this allele. (J:226871)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dstyk Mutation: 72 strains or lines available

References

• Original: J:226871 Li K, et al., DSTYK kinase domain ablation impaired the mice capabilities of learning and memory in water maze test. Int J Clin Exp Pathol. 2014;7(10):6486-92
• All: 1 reference(s)