Brd4^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Brd4^{tm1c(EUCOMM)Wtsi}
• Name: bromodomain containing 4; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6120905
• Synonyms: Brd4^fl
• Gene: Brd4 Location: Chr17:32415248-32503696 bp, - strand Genetic Position: Chr17, 17.39 cM, cytoband B2
• Alliance: Brd4^{tm1c(EUCOMM)Wtsi} page
• IMPC: Brd4 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:254909
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 32442817 of Chromosome 17 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 32443674. The critical exon(s) is/are thus flanked by loxP sites.A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:254909)

Expression

In Mice Carrying this Mutation:

29 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Brd4 Mutation: 105 strains or lines available

References

• Original: J:254909 Lee JE, et al., Brd4 binds to active enhancers to control cell identity gene induction in adipogenesis and myogenesis. Nat Commun. 2017 Dec 20;8(1):2217
• All: 5 reference(s)