App^tm1.1Aduci
Targeted Allele Detail

Summary

• Symbol: App^tm1.1Aduci
• Name: amyloid beta precursor protein; targeted mutation 1.1, Frank LaFerla
• MGI ID: MGI:6120494
• Synonyms: hABeta-KI
• Gene: App Location: Chr16:84751236-84972187 bp, - strand Genetic Position: Chr16, 46.92 cM, cytoband C3-qter
• Alliance: App^tm1.1Aduci page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:304519
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence)
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: The targeting construct includes a loxP site upstream of exon 14, 3 point mutations inserted into exon 14 to create amino acid substitutions (amino acids 5 (G->R), 10 (F->Y) and 13 (R->H)) (ENSMUSE00000131684), a loxP site downstream of exon 14 and a FRT-flanked neo selection cassette, which is removed by FLP-mediated recombination. (J:304519)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any App Mutation: 89 strains or lines available

References

• Original: J:304519 Baglietto-Vargas D, et al., Generation of a humanized Abeta expressing mouse demonstrating aspects of Alzheimer's disease-like pathology. Nat Commun. 2021 Apr 23;12(1):2421
• All: 3 reference(s)