Cluh^tm1.2Eir
Targeted Allele Detail

Summary

• Symbol: Cluh^tm1.2Eir
• Name: clustered mitochondria homolog; targeted mutation 1.2, Elena I Rugarli
• MGI ID: MGI:6119581
• Gene: Cluh Location: Chr11:74540321-74561673 bp, + strand Genetic Position: Chr11, 45.76 cM, cytoband B4
• Alliance: Cluh^tm1.2Eir page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249661
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into intron 9 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 10. The neo cassette was removed through subsequent flp-mediated recombination and exon 10 was deleted through subsequent cre-mediated recombination. Western blots confirmed the absence of protein expression from this null allele. (J:249661)

Expression

In Mice Carrying this Mutation:	112 assay results
In Structures Affected by this Mutation:	2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cluh Mutation: 48 strains or lines available

References

• Original: J:249661 Schatton D, et al., CLUH regulates mitochondrial metabolism by controlling translation and decay of target mRNAs. J Cell Biol. 2017 Mar 06;216(3):675-693
• All: 2 reference(s)