Pkn1^tm1.1Hmuk
Targeted Allele Detail

Summary

• Symbol: Pkn1^tm1.1Hmuk
• Name: protein kinase N1; targeted mutation 1.1, Hideyuki Mukai
• MGI ID: MGI:6118985
• Synonyms: PKN1 T778A
• Gene: Pkn1 Location: Chr8:84393165-84425808 bp, - strand Genetic Position: Chr8, 40.22 cM
• Alliance: Pkn1^tm1.1Hmuk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:256578
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Hypomorph)
• Mutations: Insertion, Single point mutation
• Mutation details: A T to A mutation was introduced at nucleotide 778 in exon 19 and a floxed neo cassette was inserted in the untranslated region after exon 22 via homologous recombination. Cre mediated recombination removed the neo cassette. Immunoblot analysis indicates reduced protein levels in tissues of homozygous mice. Kinase activity is absent from the mutant protein. (J:256578)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Pkn1 Mutation: 45 strains or lines available

References

• Original: J:256578 Mashud R, et al., Impaired lymphocyte trafficking in mice deficient in the kinase activity of PKN1. Sci Rep. 2017 Aug 9;7(1):7663
• All: 1 reference(s)