Ccbe1^tm1.2Dschu
Targeted Allele Detail

Summary

• Symbol: Ccbe1^tm1.2Dschu
• Name: collagen and calcium binding EGF domains 1; targeted mutation 1.2, Dorte Schulte
• MGI ID: MGI:6117810
• Synonyms: CCBE1DeltaEGF
• Gene: Ccbe1 Location: Chr18:66189926-66424909 bp, - strand Genetic Position: Chr18, 39.13 cM, cytoband E1
• Alliance: Ccbe1^tm1.2Dschu page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:249238
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Not Applicable)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 4-5 were flanked by loxP sites and a FRT-flanked neomycin resistance cassette was inserted upstream of exon 4. Flpe-mediated recombination removed the neomycin cassette. Cre-mediated recombination led to an in-frame deletion of exon 4 and 5, removing both EGF domains. Sequencing of cDNA indicates correct splicing from exon 3 to 6. (J:249238)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ccbe1 Mutation: 22 strains or lines available

References

• Original: J:249238 Roukens MG, et al., Functional Dissection of the CCBE1 Protein: A Crucial Requirement for the Collagen Repeat Domain. Circ Res. 2015 May 8;116(10):1660-9
• All: 1 reference(s)