Cd180^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Cd180^{tm1c(KOMP)Wtsi}
• Name: CD180 antigen; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6117108
• Gene: Cd180 Location: Chr13:102830055-102843139 bp, + strand Genetic Position: Chr13, 54.92 cM
• Alliance: Cd180^{tm1c(KOMP)Wtsi} page
• IMPC: Cd180 gene page

Mutation origin

• Mutant Cell Line: EPD0195_5_D11
• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 102840780 of Chromosome 13 upstream of exon 3 (Build GRCm39). The cassette contains an FRT site, lacZ coding sequence, loxP site, human beta-actin promoter controlling a neomycin resistance gene, SV40 polyA, a second FRT site and a second loxP site, was inserted upstream of exon 3. Another loxP site was inserted downstream of exon 3 at position 102843438. Flp-mediated recombination removed the FRT-flanked lacZ neo cassette, leaving exon 3 floxed. Exon 3 encodes the majority of the coding sequence for the gene. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cd180 Mutation: 50 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 3 reference(s)