Crh^{tm1.1(flpo)Bsab}
Targeted Allele Detail

Summary

• Symbol: Crh^{tm1.1(flpo)Bsab}
• Name: corticotropin releasing hormone; targeted mutation 1.1, Bernardo Sabatini
• MGI ID: MGI:6116854
• Synonyms: Crh-ires-FlpO
• Gene: Crh Location: Chr3:19747565-19749560 bp, - strand Genetic Position: Chr3, 5.75 cM
• Alliance: Crh^{tm1.1(flpo)Bsab} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6J)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Crh^{tm1.1(flpo)Bsab} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Crh (MGI:88496)

• Mutation details: The coding sequences for an an internal ribosomal entry site (IRES) followed by an optimized FLP recombinase and a self-excising lox2372-flanked neomycin-Ace-cre cassette are introduced immediately after the Stop codon. In situ characterization demonstrates that flpo and Crh expression completely overlap in the pontine micturition center (PMC) in the pons. (J:101977)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Crh (mouse) Summary of all recombinase alleles driven by Crh. Comparative matrix view of recombinase activities Alliance mouse Crh gene page

Expression

In Mice Carrying this Mutation:

11 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Crh Mutation: 42 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 4 reference(s)