Pabpc1^tm1.2Rbd
Targeted Allele Detail

Summary

• Symbol: Pabpc1^tm1.2Rbd
• Name: poly(A) binding protein, cytoplasmic 1; targeted mutation 1.2, Robert B Darnell
• MGI ID: MGI:6116824
• Synonyms: cTag-PABP, Pabpc1^cTag
• Gene: Pabpc1 Location: Chr15:36595902-36609825 bp, - strand Genetic Position: Chr15, 14.51 cM, cytoband C
• Alliance: Pabpc1^tm1.2Rbd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:250402
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Targeted (Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The initial targeting event inserted loxP site 3 to the endogenous exons 14-15, a synthetic poly(A) site preceding a FRT-Neo-FRT cassette, a second loxP site, and a second copy of exon 14 (the last coding exon of the gene) fused to AcGFP sequence. FLPmediated recombination removed the neo selection cassette, and Cre-mediated recombination removed the endogenous exons 14 and 15, leaving the second copy of exon 14 (the last coding exon of the gene) fused to AcGFP sequence. (J:250402)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Pabpc1 Mutation: 34 strains or lines available

References

• Original: J:250402 Hwang HW, et al., cTag-PAPERCLIP Reveals Alternative Polyadenylation Promotes Cell-Type Specific Protein Diversity and Shifts Araf Isoforms with Microglia Activation. Neuron. 2017 Sep 13;95(6):1334-1349.e5
• All: 3 reference(s)