Gt(ROSA)26Sor^{tm1(CAG-COP4*E123A/EGFP)Ylu}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-COP4*E123A/EGFP)Ylu}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Youming Lu
• MGI ID: MGI:6115610
• Synonyms: ChR2^loxP, Rosa-CAG-Flag-ChR2eGFP-WPRE
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-COP4*E123A/EGFP)Ylu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236450
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Epitope tag, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector contains a CAG (CMV-IE enhancer/chicken, beta-actin/rabbit-globin hybrid promoter), an FRT site, a loxP-flanked STOP cassette, a FLAG-tagged EGFP reporter gene fused to the channelrhodopsin-2 from the green alga Chlamydomonas reinhardtii modified to contain a E123A substitution (COP4*E123A), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a poly-A signal, and an attB/att-flanked PGK-FRT-Neo-poly-A cassette. (J:236450)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:236450 Shu S, et al., Selective Degeneration of Entorhinal-CA1 Synapses in Alzheimer's Disease via Activation of DAPK1. J Neurosci. 2016 Oct 19;36(42):10843-10852
• All: 3 reference(s)