Dppa3^em1Dean
Endonuclease-mediated Allele Detail

Summary

• Symbol: Dppa3^em1Dean
• Name: developmental pluripotency-associated 3; endonuclease-mediated mutation 1, Jurrien Dean
• MGI ID: MGI:6115592
• Synonyms: Dppa3^R60A
• Gene: Dppa3 Location: Chr6:122603383-122607230 bp, + strand Genetic Position: Chr6, 57.72 cM
• Alliance: Dppa3^em1Dean page

Mutation origin

• Strain of Origin: (C57BL/6 x DBA/2)F1

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: CRISPR targeting produced a ACGG to GGCC nucleotide substitution that results in the amino acid substitution of alanine for arginine at position 60 (R60A). This mutation abolishes cytoplasmic cleavage. (J:256230)

Expression

In Mice Carrying this Mutation:

2 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dppa3 Mutation: 23 strains or lines available

References

• Original: J:256230 Shin SW, et al., Cytoplasmic cleavage of DPPA3 is required for intracellular trafficking and cleavage-stage development in mice. Nat Commun. 2017 Nov 21;8(1):1643
• All: 1 reference(s)