Ngly1^{tm2d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ngly1^{tm2d(KOMP)Wtsi}
• Name: N-glycanase 1; targeted mutation 2d, Wellcome Trust Sanger Institute
• MGI ID: MGI:6114996
• Gene: Ngly1 Location: Chr14:6157837-6220449 bp, - strand Genetic Position: Chr14, 7.08 cM, cytoband A2
• Alliance: Ngly1^{tm2d(KOMP)Wtsi} page
• IMPC: Ngly1 gene page

Mutation origin

• Mutant Cell Line: EPD0044_5_F07
• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_gt2
• Mutation details: The L1L2_gt2 cassette was placed upstream of the critical exon (exon 2) of the gene; inserting at bp position Chr14:16,254,154 [Build GRCm38]. The cassette is composed of composed of an FRT site, a mouse En2 intron/splice-acceptor/exon (En2 SA), a self-cleaving 2A peptide, the beta-galactosidase gene (lacZ), a second self-cleaving 2A peptide, the neomycin-resistance gene (neo), an SV40 polyadenylation signal (SV40pA), a second FRT site and a loxP site. A second loxP site was inserted downstream of exon 2 at bp position Chr14:16,255,007 bp [Build GRCm38]; thereby flanking exon 2 with loxP sites. Cre-mediated recombination removed exon 2. Flp-mediated recombination removed the FRT-flanked neo cassette (J:101977, J:148605)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ngly1 Mutation: 40 strains or lines available

References

• Original: J:148605 Wellcome Trust Sanger Institute, Alleles produced for the KOMP project by the Wellcome Trust Sanger Institute. MGI Direct Data Submission. 2009
• All: 2 reference(s)