Cdkn2a^em2Cjs
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cdkn2a^em2Cjs
• Name: cyclin dependent kinase inhibitor 2A; endonuclease-mediated mutation 2, Charles J Sherr
• MGI ID: MGI:6113939
• Synonyms: p19^Arf-M45A
• Gene: Cdkn2a Location: Chr4:89192710-89212856 bp, - strand Genetic Position: Chr4, 42.15 cM, cytoband C3-C6
• Alliance: Cdkn2a^em2Cjs page

Mutation origin

• Strain of Origin: C57BL/6J x C57BL/6N

Mutation description

• Allele Type: Endonuclease-mediated (Modified isoform(s))
• Mutation: Intragenic deletion
• Mutation details: For CRSIPR/Cas9 mutagenesis, C57BL/6 ES cells were transfected with an all-in-one construct expressing Cas9 and an sgRNA targeting the internal Arf translation initiation codon at position 45 (shown in capital letters; 5'-ctttcgtgaacATGttgttg-3'), and a 127-base single-strand oligodeoxynucleotide (ssODN) template. The template contains, between homology arms, 5'-ctttcgtgaacGCCCtgttgCGC-3'. The template directs the mutation of the downstream ATG codon to GCC (p.Met45Ala), the disruption of the sgRNA-targeting site through a T>C substitution, and the convertion of the 3' PAM sequence from AGG to CGC (all mutations shown in capital letters in the DNA sequence above). The latter two mutations do not affect the sequence of the encoded peptide. (J:244260)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Cdkn2a Mutation: 62 strains or lines available

References

• Original: J:244260 van Oosterwijk JG, et al., Small mitochondrial Arf (smArf) protein corrects p53-independent developmental defects of Arf tumor suppressor-deficient mice. Proc Natl Acad Sci U S A. 2017 Jul 11;114(28):7420-7425
• All: 1 reference(s)