Col1a1^{tm1.1(tetO-mStrawberry/Atg4b*C74A)Kmln}
Targeted Allele Detail

Summary

• Symbol: Col1a1^{tm1.1(tetO-mStrawberry/Atg4b*C74A)Kmln}
• Name: collagen, type I, alpha 1; targeted mutation 1.1, Bjorn Knollmann
• MGI ID: MGI:6111715
• Synonyms: ATG4B^C74A, ATG4B^CA
• Gene: Col1a1 Location: Chr11:94827050-94843868 bp, + strand Genetic Position: Chr11, 59.01 cM, cytoband D
• Alliance: Col1a1^{tm1.1(tetO-mStrawberry/Atg4b*C74A)Kmln} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:256651
• Parent Cell Line: C2 (Nagy) (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Inducible, Inserted expressed sequence, Reporter)
• Inducer: doxycycline/tetracycline
• Mutations: Insertion, Nucleotide substitutions
• Col1a1^{tm1.1(tetO-mStrawberry/Atg4b*C74A)Kmln} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Atg4b (MGI:1913865)	fused to mStrawberry and contains aa substitution C74A

• Col1a1^{tm1.1(tetO-mStrawberry/Atg4b*C74A)Kmln} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  Not Specified	tetO

• Mutation details: The construct contains an mStrawberry red fluorescence protein fused to a modified Atg4b gene under control of a tet-responsive element (TRE; tetO). In this construct, Atg4b contains a dominant negative C74A mutation, which results in the expression of an enzymatically inactive protein. This mStrawberry/Atg4B cDNA fragment was co-electroporated into C57BL/6N-derived C2 embryonic stem (ES) cells along with a pCol-TGM targeting vector, containing an FRT-flanked PGK-Neo inserted into the 3' UTR of the Col1a1 locus, and a pCAGGS-FlpE expression plasmid. (J:256651)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Col1a1 Mutation: 166 strains or lines available

References

• Original: J:256651 Yang A, et al., Autophagy Sustains Pancreatic Cancer Growth through Both Cell-Autonomous and Nonautonomous Mechanisms. Cancer Discov. 2018 Mar;8(3):276-287
• All: 1 reference(s)