Exosc10^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Exosc10^{tm1c(KOMP)Wtsi}
• Name: exosome component 10; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:6110581
• Synonyms: Exosc10^f
• Gene: Exosc10 Location: Chr4:148642886-148666858 bp, + strand Genetic Position: Chr4, 78.76 cM
• Alliance: Exosc10^{tm1c(KOMP)Wtsi} page
• IMPC: Exosc10 gene page

Mutation origin

• Mutant Cell Lines: EPD0567_5_B04, EPD0567_5_F03
• Germline Transmission: Earliest citation of germline transmission: J:253584
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 148645015 of Chromosome 4 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 148645831. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:253584)

Disease models

Expression

In Structures Affected by this Mutation:

7 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Exosc10 Mutation: 61 strains or lines available

References

• Original: J:253584 Jamin SP, et al., EXOSC10/Rrp6 is post-translationally regulated in male germ cells and controls the onset of spermatogenesis. Sci Rep. 2017 Nov 8;7(1):15065
• All: 2 reference(s)