Gt(ROSA)26Sor^{tm1(CAG-Lef1,-GFP*)Mahll}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-Lef1,-GFP*)Mahll}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Melinda Angus-Hill
• MGI ID: MGI:6108866
• Synonyms: ROSA^Lef1GFP
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-Lef1,-GFP*)Mahll} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-Lef1,-GFP*)Mahll} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Lef1 (MGI:96770)

• Mutation details: The targeting vector is designed with (from 5' to 3') a ubiquitously expressed CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), loxP-flanked stop cassette, murine lymphoid enhancer binding factor 1 (Lef1) cDNA, a membrane bound T2-Green Fluorescent Protein (T2-mGFP), and a neomycin resistance (neo) cassette inserted into the Gt(ROSA)26Sor locus. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

Notes

ES cell line = G456, a derivative of ES cell line G4.

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)