Gt(ROSA)26Sor^{tm1.1(HBEGF)Iiis}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(HBEGF)Iiis}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Masashi Yanagisawa and Hiromasa Funato
• MGI ID: MGI:6107764
• Synonyms: Gt(ROSA)26Sor^{tm1(DTR-tdTomato)Iiis}, Rosa-DTR-Tomato
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(HBEGF)Iiis} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:263766
• Parent Cell Line: RENKA (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1.1(HBEGF)Iiis} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  monkey	HBEGF

• Mutation details: The targeting construct inserted a slice acceptor, loxP site, two polyadenylation sequences, an FRT-flanked neomycin resistance cassette, a STOP cassette, a second loxP site, a simian diphtheria toxin receptor (heparin- binding epidermal growth factor-like growth factor), IRES sequence, tdTomato reporter gene and polyadenylation sequence. Flp-mediated recombination removed the resistance cassette but left the STOP cassette floxed. (J:263766)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:263766 Iwasaki K, et al., Ablation of Central Serotonergic Neurons Decreased REM Sleep and Attenuated Arousal Response. Front Neurosci. 2018;12:535
• All: 3 reference(s)