Omp^tm2.1Tboz
Targeted Allele Detail

Summary

• Symbol: Omp^tm2.1Tboz
• Name: olfactory marker protein; targeted mutation 2.1, Thomas Bozza
• MGI ID: MGI:6107198
• Synonyms: Omp^{tm1(GCaMP3)Tboz}
• Gene: Omp Location: Chr7:97792566-97794654 bp, - strand Genetic Position: Chr7, 53.59 cM
• Alliance: Omp^tm2.1Tboz page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The coding sequence of GCaMP3 (a GFP-based calcium sensor for imaging calcium dynamics) followed by a self-excising neomycin selection cassette replaces the coding sequence of the mouse gene. The GCaMP3 fusion gene contains a cDNA sequence encoding the chicken smooth muscle M13 fragment of myosin light chain kinase, a circularly permutated EGFP (amino acid residues 149-238 followed by amino acid residues 1-144 (including M153K, V163A, S175G, D180Y, T203V, A206K, and V93I mutations to increase dynamic range and baseline fluorescence), and a DNA fragment encoding amino acid residues 2-148 of the rat calmodulin (modified to harbor the N60D mutation to increase the fluorescence change for small calcium transients). (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Omp Mutation: 26 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 5 reference(s)