Wdr47^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Wdr47^{tm1b(EUCOMM)Wtsi}
• Name: WD repeat domain 47; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:6102912
• Gene: Wdr47 Location: Chr3:108498595-108553035 bp, + strand Genetic Position: Chr3, 47.38 cM
• Alliance: Wdr47^{tm1b(EUCOMM)Wtsi} page
• IMPC: Wdr47 gene page

Mutation origin

• Mutant Cell Line: EPD0046_1_C04
• Germline Transmission: Earliest citation of germline transmission: J:249035
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_gt0
• Mutation details: The L1L2_gt0 cassette was inserted at position 108525343 of Chromosome 3 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 108526974. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). (J:249035)

Expression

In Mice Carrying this Mutation:

25 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Wdr47 Mutation: 52 strains or lines available

References

• Original: J:249035 Kannan M, et al., WD40-repeat 47, a microtubule-associated protein, is essential for brain development and autophagy. Proc Natl Acad Sci U S A. 2017 Oct 31;114(44):E9308-E9317
• All: 2 reference(s)