Chrna1^{tm1d(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Chrna1^{tm1d(EUCOMM)Hmgu}
• Name: cholinergic receptor nicotinic alpha 1 subunit; targeted mutation 1d, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6101516
• Synonyms: Chrna1^delta4
• Gene: Chrna1 Location: Chr2:73393625-73410682 bp, - strand Genetic Position: Chr2, 43.76 cM
• Alliance: Chrna1^{tm1d(EUCOMM)Hmgu} page
• IMPC: Chrna1 gene page

Mutation origin

• Mutant Cell Line: HEPD0507_1_B05
• Germline Transmission: Earliest citation of germline transmission: J:244196
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 73403361 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 73404136. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked critical exon(s). (J:244196)

Expression

In Mice Carrying this Mutation:

9 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chrna1 Mutation: 17 strains or lines available

References

• Original: J:244196 Roux I, et al., Assessment of the expression and role of the alpha1-nAChR subunit in efferent cholinergic function during the development of the mammalian cochlea. J Neurophysiol. 2016 Aug 01;116(2):479-92
• All: 1 reference(s)