Ino80^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ino80^{tm1c(EUCOMM)Hmgu}
• Name: INO80 complex subunit; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:6100919
• Synonyms: Ino80^fl
• Gene: Ino80 Location: Chr2:119203523-119308168 bp, - strand Genetic Position: Chr2, 59.97 cM, cytoband E5
• Alliance: Ino80^{tm1c(EUCOMM)Hmgu} page
• IMPC: Ino80 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:230583
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 119281571 of Chromosome 2 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 119282378. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:230583)

Expression

In Mice Carrying this Mutation:

4 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ino80 Mutation: 438 strains or lines available

References

• Original: J:230583 Serber DW, et al., The Mouse INO80 Chromatin-Remodeling Complex Is an Essential Meiotic Factor for Spermatogenesis. Biol Reprod. 2016 Dec;94(1):8
• All: 5 reference(s)