Tuba8^tm1.2Ctpd
Targeted Allele Detail

Summary

• Symbol: Tuba8^tm1.2Ctpd
• Name: tubulin, alpha 8; targeted mutation 1.2, Christine P Diggle
• MGI ID: MGI:6094271
• Gene: Tuba8 Location: Chr6:121187655-121203813 bp, + strand Genetic Position: Chr6, 57.15 cM, cytoband F1
• Alliance: Tuba8^tm1.2Ctpd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:248203
• Parent Cell Line: E14.1 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: An FRT-flanked neomycin with a 5' lox site was inserted upstream of exon 3. An additional loxP site was inserted downstream of exon 3. Flp-mediated recombination removed the selection cassette. Cre-mediated recombination removed exon 3. Immunohistochemistry confirmed the absence of protein expression in the testes and brain. (J:248203)

Disease models

Expression

In Mice Carrying this Mutation:

38 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Tuba8 Mutation: 28 strains or lines available

References

• Original: J:248203 Diggle CP, et al., A tubulin alpha 8 mouse knockout model indicates a likely role in spermatogenesis but not in brain development. PLoS One. 2017;12(4):e0174264
• All: 1 reference(s)