Slc9a8^{tm1d(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Slc9a8^{tm1d(KOMP)Wtsi}
• Name: solute carrier family 9 (sodium/hydrogen exchanger), member 8; targeted mutation 1d, Wellcome Trust Sanger Institute
• MGI ID: MGI:5925354
• Synonyms: Nhe8^-
• Gene: Slc9a8 Location: Chr2:167263632-167318920 bp, + strand Genetic Position: Chr2, 87.22 cM
• Alliance: Slc9a8^{tm1d(KOMP)Wtsi} page
• IMPC: Slc9a8 gene page

Mutation origin

• Mutant Cell Line: EPD0033_5_A07
• Germline Transmission: Earliest citation of germline transmission: J:245977
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_gt1
• Mutation details: The L1L2_gt1 cassette was inserted at position 167282591 of Chromosome 2 upstream of the critical exon 4 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 4 at position 167283331. Exon 4 is thus flanked by loxP sites. A null/knockout allele was created by flp and cre recombinase expression in mice carrying this allele to remove the lacZ sequence, neo selection cassette and the loxP-flanked exon 4. Western blot analysis confirmed the absence of protein expression in the male and female reproductive system and kidney of adult homozygous mutant mice. (J:245977)

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Slc9a8 Mutation: 110 strains or lines available

References

• Original: J:245977 Oberheide K, et al., Loss of the Na+/H+ exchanger NHE8 causes male infertility in mice by disrupting acrosome formation. J Biol Chem. 2017 Jun 30;292(26):10845-10854
• All: 2 reference(s)