Ttc8^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Ttc8^{tm1c(KOMP)Wtsi}
• Name: tetratricopeptide repeat domain 8; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5925339
• Synonyms: Bbs8^flox
• Gene: Ttc8 Location: Chr12:98886833-98949497 bp, + strand Genetic Position: Chr12, 49.88 cM
• Alliance: Ttc8^{tm1c(KOMP)Wtsi} page
• IMPC: Ttc8 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:246062
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 98909097 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 98910435. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:246062)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ttc8 Mutation: 35 strains or lines available

References

• Original: J:246062 Hsu Y, et al., BBSome function is required for both the morphogenesis and maintenance of the photoreceptor outer segment. PLoS Genet. 2017 Oct;13(10):e1007057
• All: 5 reference(s)