Septin12^tm2.1Plk
Targeted Allele Detail

Summary

• Symbol: Septin12^tm2.1Plk
• Name: septin 12; targeted mutation 2.1, Pao-Lin Kuo
• MGI ID: MGI:5919980
• Synonyms: SEPT12^S196E
• Gene: Septin12 Location: Chr16:4804722-4815716 bp, - strand Genetic Position: Chr16, 2.49 cM, cytoband A1
• Alliance: Septin12^tm2.1Plk page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242315
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Dominant negative)
• Mutation: Nucleotide substitutions
• Mutation details: Substitutions (c.196AGC>GAG) were engineered in codon 196 in exon 6, changing it from a serine to a glutamate codon (p.Ser196Glu or p.S196E). A loxP site flanked neomycin resistance gene was inserted into intron 7. the neo cassette was removed through subsequent cre-mediated recombination. In the peptide the mutation acts as a phosphomimetic. (J:242315)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Septin12 Mutation: 25 strains or lines available

References

• Original: J:242315 Shen YR, et al., SEPT12 phosphorylation results in loss of the septin ring/sperm annulus, defective sperm motility and poor male fertility. PLoS Genet. 2017 Mar;13(3):e1006631
• All: 1 reference(s)