Gt(ROSA)26Sortm1(CAG-MCPySTco)Cvp
Targeted Allele Detail
|
Symbol: |
Gt(ROSA)26Sortm1(CAG-MCPySTco)Cvp |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Patrick Moore |
MGI ID: |
MGI:5911880 |
Synonyms: |
Gt(ROSA)26SorTg(CAG-MCPyV_gp4)Cvp, ROSA26-CAG-LNL-MCVsTco, ROSA26sT, ROSAsT |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
|
Alliance: |
Gt(ROSA)26Sortm1(CAG-MCPySTco)Cvp page
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:243752
|
Parent Cell Line: |
E14 (ES Cell)
|
Strain of Origin: |
129P2/OlaHsd
|
|
Allele Type: |
|
Targeted (Conditional ready, Inserted expressed sequence) |
Mutation: |
|
Insertion
|
|
|
Mutation details: Merkel cell polyomavirus (MCPyV), a close relative of simian vacuolating 40 (SV40) virus, is clonally integrated into the tumor genome in ~80% of Merkel cell carcinomas (MCC); MCC is a highly aggressive non-melanoma skin cancer. The knocked-in sequence of this allele contains the CAG promoter - which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene - followed by a loxP-flanked STOP/Pgk-neomycin phosphotransferase (neo) cassette and a codon-optimized cDNA encoding the small T antigen of MCPyV (MCPySTco; also called MCPyV_gp4, protein sequence YP_009111422.1) with the human growth hormone polyadenylation signal. Upon tissue-specific Cre recombinase excision of the STOP/neo cassette, expression of the small T antigen is driven by the CAG promoter.
(J:243752)
|
|
|
Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
|
Carrying any Gt(ROSA)26Sor Mutation: |
944 strains or lines available
|
|
Original: |
J:243752 Shuda M, et al., Merkel Cell Polyomavirus Small T Antigen Induces Cancer and Embryonic Merkel Cell Proliferation in a Transgenic Mouse Model. PLoS One. 2015;10(11):e0142329 |
All: |
3 reference(s) |
|