Gt(ROSA)26Sor^{tm1(CAG-MCPySTco)Cvp}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-MCPySTco)Cvp}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Patrick Moore
• MGI ID: MGI:5911880
• Synonyms: Gt(ROSA)26Sor^{Tg(CAG-MCPyV_gp4)Cvp}, ROSA26-CAG-LNL-MCVsTco, ROSA26^sT, ROSA^sT
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-MCPySTco)Cvp} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243752
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details: Merkel cell polyomavirus (MCPyV), a close relative of simian vacuolating 40 (SV40) virus, is clonally integrated into the tumor genome in ~80% of Merkel cell carcinomas (MCC); MCC is a highly aggressive non-melanoma skin cancer. The knocked-in sequence of this allele contains the CAG promoter - which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene - followed by a loxP-flanked STOP/Pgk-neomycin phosphotransferase (neo) cassette and a codon-optimized cDNA encoding the small T antigen of MCPyV (MCPySTco; also called MCPyV_gp4, protein sequence YP_009111422.1) with the human growth hormone polyadenylation signal. Upon tissue-specific Cre recombinase excision of the STOP/neo cassette, expression of the small T antigen is driven by the CAG promoter. (J:243752)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 945 strains or lines available

References

• Original: J:243752 Shuda M, et al., Merkel Cell Polyomavirus Small T Antigen Induces Cancer and Embryonic Merkel Cell Proliferation in a Transgenic Mouse Model. PLoS One. 2015;10(11):e0142329
• All: 3 reference(s)