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Gt(ROSA)26Sortm1(CAG-MCPySTco)Cvp
Targeted Allele Detail
Summary
Symbol: Gt(ROSA)26Sortm1(CAG-MCPySTco)Cvp
Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Patrick Moore
MGI ID: MGI:5911880
Synonyms: Gt(ROSA)26SorTg(CAG-MCPyV_gp4)Cvp, ROSA26-CAG-LNL-MCVsTco, ROSA26sT, ROSAsT
Gene: Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance: Gt(ROSA)26Sortm1(CAG-MCPySTco)Cvp page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:243752
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Conditional ready, Inserted expressed sequence)
Mutation:    Insertion
 
Mutation detailsMerkel cell polyomavirus (MCPyV), a close relative of simian vacuolating 40 (SV40) virus, is clonally integrated into the tumor genome in ~80% of Merkel cell carcinomas (MCC); MCC is a highly aggressive non-melanoma skin cancer. The knocked-in sequence of this allele contains the CAG promoter - which consists of the cytomegalovirus immediate early (CMV-EI) enhancer followed by a 1.3-kb DNA segment including the promoter, first exon and first intron of the chicken beta-actin gene, with the 3' splice junction sequence replaced by that of the rabbit hemoglobin beta gene - followed by a loxP-flanked STOP/Pgk-neomycin phosphotransferase (neo) cassette and a codon-optimized cDNA encoding the small T antigen of MCPyV (MCPySTco; also called MCPyV_gp4, protein sequence YP_009111422.1) with the human growth hormone polyadenylation signal. Upon tissue-specific Cre recombinase excision of the STOP/neo cassette, expression of the small T antigen is driven by the CAG promoter. (J:243752)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:  944 strains or lines available
References
Original:  J:243752 Shuda M, et al., Merkel Cell Polyomavirus Small T Antigen Induces Cancer and Embryonic Merkel Cell Proliferation in a Transgenic Mouse Model. PLoS One. 2015;10(11):e0142329
All:  3 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory