Etl4^tm2.1Gos
Targeted Allele Detail

Summary

• Symbol: Etl4^tm2.1Gos
• Name: enhancer trap locus 4; targeted mutation 2.1, Achim Gossler
• MGI ID: MGI:5911706
• Synonyms: Skt^Ex5lacZ
• Gene: Etl4 Location: Chr2:19915018-20815346 bp, + strand Genetic Position: Chr2, 14.27 cM, cytoband A3
• Alliance: Etl4^tm2.1Gos page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229383
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Modified isoform(s), Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A lacZ with a triple poly A signal and a floxed pgk-neo cassette replaced a 695 bp region including part of exon 5 and intron 5. Cre-mediated recombination removed the neo cassette. RT-PCR detected the presence of an alternative splice form with exon 4 fused to exon 6, skipping the targeted exon, resulting in an in frame deletion of 96 amino acids. LacZ expression is detected in the notochord, the optic vesicle, the otic placode, the eye, the apical ectodermal ridge of the fore and hindlimbs and on the surface of the branchial arches at E10.5 and E11.5. (J:229383)

Expression

In Mice Carrying this Mutation:

30 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Etl4 Mutation: 485 strains or lines available

References

• Original: J:229383 Serth K, et al., Generation of an 870 kb deletion encompassing the Skt/Etl4 locus by combination of inter- and intra-chromosomal recombination. BMC Genet. 2015;16:143
• All: 1 reference(s)