Hoxb5^em1.1Ilw
Endonuclease-mediated Allele Detail

Summary

• Symbol: Hoxb5^em1.1Ilw
• Name: homeobox B5; endonuclease-mediated mutation 1.1, Irving L Weissman
• MGI ID: MGI:5911679
• Synonyms: Hoxb5-tri-mCherry
• Gene: Hoxb5 Location: Chr11:96194338-96196947 bp, + strand Genetic Position: Chr11, 59.82 cM
• Alliance: Hoxb5^em1.1Ilw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:229236
• Parent Cell Line: Bruce 4 (ES Cell)
• Strain of Origin: B6.Cg-Thy1^a

Mutation description

• Allele Type: Endonuclease-mediated (No functional change, Reporter)
• Mutation: Insertion
• Mutation details: Three tandem mCherry cassettes separated by porcine teschovirus-1 2A (P2A) sequences and a floxed pgk-neo cassette were inserted immediately 5' of the stop codon in exon 2. The terminal mCherry cassette includes a CAAX membrane localization sequence. Cre-mediated recombination removed the neo cassette. (J:229236)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxb5 Mutation: 11 strains or lines available

References

• Original: J:229236 Chen JY, et al., Hoxb5 marks long-term haematopoietic stem cells and reveals a homogenous perivascular niche. Nature. 2016 Feb 11;530(7589):223-7
• All: 2 reference(s)