Mapt^{tm1(lacZ,HBEGF/EGFP)Gld}
Targeted Allele Detail

Summary

• Symbol: Mapt^{tm1(lacZ,HBEGF/EGFP)Gld}
• Name: microtubule-associated protein tau; targeted mutation 1, Martyn Goulding
• MGI ID: MGI:5911677
• Synonyms: Mapt^ds-DTR, Tau^ds-DTR
• Gene: Mapt Location: Chr11:104122216-104222916 bp, + strand Genetic Position: Chr11, 67.79 cM, cytoband E1
• Alliance: Mapt^{tm1(lacZ,HBEGF/EGFP)Gld} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227180
• Parent Cell Line: 2A (ES Cell)
• Strain of Origin: 129S1/SvImJ

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mapt^{tm1(lacZ,HBEGF/EGFP)Gld} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  monkey	HBEGF

• Mutation details: Sequences encoding for the simian diphtheria toxin receptor (DTR) fused to EGFP and bGH polyA sequences downstream of a double-stop (ds) cassette consisting of a floxed STOP signal and an frt flanked nuclear bata-galactosidase (nlsLacZ) and STOP signal was inserted into Mapt locus. Endogenous Mapt promoter drives expression of nlsLacZ or DTR/EGFP fusion protein. Cre mediated removal of the first STOP signal is required for expression of nlsLacZ. Cre and flp mediated recombination are required to remove both STOP signals to allow for expression of the DTR/EGFP fusion protein. (J:227180)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mapt Mutation: 453 strains or lines available

References

• Original: J:227180 Britz O, et al., A genetically defined asymmetry underlies the inhibitory control of flexor-extensor locomotor movements. Elife. 2015;4
• All: 23 reference(s)