Ins1^em1(cre)Utr
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ins1^em1(cre)Utr
• Name: insulin I; endonuclease-mediated mutation 1, Ken-ichi Yagami
• MGI ID: MGI:5911527
• Gene: Ins1 Location: Chr19:52252735-52253453 bp, + strand Genetic Position: Chr19, 46.76 cM
• Alliance: Ins1^em1(cre)Utr page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Recombinase)
• Mutation: Insertion
• Ins1^em1(cre)Utr expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ins1 (MGI:96572)

• Mutation details: The bicistronic Ins1-2A-Cre knock-in allele was created using homologous recombination (aided by targeted CRISPR/cas9 endonuclease activity) to insert a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping) and a Cre recombinase gene (cre) just upstream of the endogenous STOP codon of Ins1. An anealed Ins1-CRISPR-F and Ins1-CRISPR-R were inserted into the entry site of plasmid carrying both gRNA and Cas9 expression units. DNA vectors were microinjected into the pronuclei of C57BL/6J fertilized zygotes. Embryos were transferred to pseudopregnant females. (J:240225)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ins1 (mouse) Summary of all recombinase alleles driven by Ins1. Alliance mouse Ins1 gene page

Expression

In Mice Carrying this Mutation:

52018 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ins1 Mutation: 46 strains or lines available

References

• Original: J:240225 Hasegawa Y, et al., Generation of CRISPR/Cas9-mediated bicistronic knock-in ins1-cre driver mice. Exp Anim. 2016 Jul 29;65(3):319-27
• All: 4 reference(s)