Tg(tetO-Ctsb/RB1)14Smrs
Transgene Detail

Summary

• Symbol: Tg(tetO-Ctsb/RB1)14Smrs
• Name: transgene insertion 14, Sonia M Rocha-Sanchez
• MGI ID: MGI:5911114
• Synonyms: DN-CBRb
• Transgene: Tg(tetO-Ctsb/RB1)14Smrs Location: unknown Genetic Position: Chr10, Syntenic
• Alliance: Tg(tetO-Ctsb/RB1)14Smrs page

Transgene origin

• Strain of Origin: 129 and C57BL/6

Transgene description

• Transgene Type: Transgenic (Dominant negative, Epitope tag, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Tg(tetO-Ctsb/RB1)14Smrs expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CTSB (1508)

  Ctsb	(MGI:88561)

  mouse

  Rb1 (MGI:97874)
• Mutation details: A 1583 bp segement of the human RB1 cDNA (bases 1243-2826, amino-acids 369-896) was cloned into the pCS2+CB-Myc6 vector. This vector contains a 1012 bp (337 amino-acids) long construct of bp 35-1047 of Ctsb with 6x Myc tags fused to its C-terminus. The resulting fusion product is an 865 aa peptide comprising Ctsb, six Myc tags and Rb1. The fusion gene was then cloned into a pTet-splice vector which brings the fusion gene under the control of the tetO promoter and terminates the gene with an SV40 intron and polyA signals. This transgenic construct was used to generate several founder mice, of which this line was used for further study. The transgene results in a tetracycline-inducibele dominant negative allele that disrupts the function of Rb1-containing multimeric complexes by shuttling them to the lysosome for degradation. (J:242452)
• Inheritance: Dominant

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:242452 Tarang S, et al., Generation of a Retinoblastoma (Rb)1-inducible dominant-negative (DN) mouse model. Front Cell Neurosci. 2015;9:52
• All: 1 reference(s)