Optn^tm1.1Arte
Targeted Allele Detail

Summary

• Symbol: Optn^tm1.1Arte
• Name: optineurin; targeted mutation 1.1, TaconicArtemis
• MGI ID: MGI:5911095
• Synonyms: Optn^D477N
• Gene: Optn Location: Chr2:5025453-5068862 bp, - strand Genetic Position: Chr2, 3.15 cM
• Alliance: Optn^tm1.1Arte page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243728
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutations: Insertion, Single point mutation
• Mutation details: A loxP site and FRT flanked neo cassette was inserted in intron 11, an Asp to Asn mutation was introduced at position 477 in exon 12, and a loxP site was inserted in intron 12 via homologous recombination. Flp-mediated recombination removed the neo cassette. The mutant protein is expressed at higher levels than the wild-type protein and is unable to bind Lys-63-linked polyubiquitin chains. (J:243728)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Optn Mutation: 48 strains or lines available

References

• Original: J:243728 Gleason CE, et al., Polyubiquitin binding to optineurin is required for optimal activation of TANK-binding kinase 1 and production of interferon beta. J Biol Chem. 2011 Oct 14;286(41):35663-74
• All: 3 reference(s)