Nlrp9b^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Nlrp9b^{tm1c(KOMP)Wtsi}
• Name: NLR family, pyrin domain containing 9B; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5910443
• Synonyms: Nlrp9b-floxed
• Gene: Nlrp9b Location: Chr7:19725318-19796867 bp, + strand Genetic Position: Chr7, 9.96 cM
• Alliance: Nlrp9b^{tm1c(KOMP)Wtsi} page
• IMPC: Nlrp9b gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243487
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 20022681 of Chromosome 7 in intron 1 (Build GRCm38). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted in intron 2 at position 20024947. Exon 2 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). (J:243487)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nlrp9b Mutation: 51 strains or lines available

References

• Original: J:243487 Zhu S, et al., Nlrp9b inflammasome restricts rotavirus infection in intestinal epithelial cells. Nature. 2017 Jun 29;546(7660):667-670
• All: 1 reference(s)