Tg(Dclk1-cre/ERT2)#Tcw
Transgene Detail

Summary

• Symbol: Tg(Dclk1-cre/ERT2)#Tcw
• Name: transgene insertion, Timothy C Wang
• MGI ID: MGI:5909916
• Synonyms: Dclk1-BAC-CreERT, Dclk1-CreERT
• Transgene: Tg(Dclk1-cre/ERT2)#Tcw Location: unknown
• Alliance: Tg(Dclk1-cre/ERT2)#Tcw page

Transgene origin

• Strain of Origin: (CBA x C57BL/6J)F1

Transgene description

• Transgene Type: Transgenic (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Tg(Dclk1-cre/ERT2)#Tcw expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Dclk1 (MGI:1330861)

• Mutation details: Using recombineering methods, the CreTM-FrtNeoFrt cassette was inserted upstream of the ATG codon in exon 2 of the Dclk1 gene in the mouse BAC clone RP23-283D6. The modified BAC construct was injected into fertilized CBA x C57BL/6J oocytes. Four founders positive for the transgene were obtained and backcrossed to C57BL/6J mice. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:209426)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Dclk1 (mouse) Summary of all recombinase alleles driven by Dclk1. Comparative matrix view of recombinase activities Alliance mouse Dclk1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:209426 Westphalen CB, et al., Long-lived intestinal tuft cells serve as colon cancer-initiating cells. J Clin Invest. 2014 Mar 3;124(3):1283-95
• All: 10 reference(s)