Apela^em1.1Hadj
Endonuclease-mediated Allele Detail

Summary

• Symbol: Apela^em1.1Hadj
• Name: apelin receptor early endogenous ligand; endonuclease-mediated mutation 1.1, Anna-Katerina Hadjantonakis
• MGI ID: MGI:5909264
• Synonyms: Apela^H2B-GFP, NEO-OUT
• Gene: Apela Location: Chr8:65481069-65489970 bp, - strand Genetic Position: Chr8, 32.52 cM
• Alliance: Apela^em1.1Hadj page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243623
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: B6(Cg)-Tyr^c-2J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Plasmids encoding a signal guide RNA are designed to knock-in a H2B-GFP-pA reporter cassette and a loxP site flanked PGK-NEO cassette into exon 2, the endogenous translational start site of the gene. Cre-mediated recombination removed the floxed neo cassette. Quantitative RT-PCR confirmed that 3' UTR was not amplified although both spliced and unspliced 5'UTR is detected. This is a null allele and a reporter allele. (J:243623)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Apela Mutation: 18 strains or lines available

Notes

Lentiviral gene trap injected into zygotes.

References

• Original: J:243623 Freyer L, et al., Loss of Apela Peptide in Mice Causes Low Penetrance Embryonic Lethality and Defects in Early Mesodermal Derivatives. Cell Rep. 2017 Aug 29;20(9):2116-2130
• All: 1 reference(s)