Ca1^{tm1.1(cre/ERT2)Cle}
Targeted Allele Detail

Summary

• Symbol: Ca1^{tm1.1(cre/ERT2)Cle}
• Name: carbonic anhydrase 1; targeted mutation 1.1, Hans Clevers
• MGI ID: MGI:5908163
• Synonyms: Car1^CreER
• Gene: Ca1 Location: Chr3:14831274-14873425 bp, - strand Genetic Position: Chr3, 3.18 cM
• Alliance: Ca1^{tm1.1(cre/ERT2)Cle} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:238627
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inducible, Recombinase)
• Inducer: tamoxifen
• Mutation: Insertion
• Ca1^{tm1.1(cre/ERT2)Cle} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Ca1 (MGI:88268)

• Mutation details: An IRES-cre/ERT2 cassette followed by a Frt-flanked neo-selection cassette was inserted into exon 7 of the Car1 gene by homologous recombination in ES cells. Correctly targeted ES cell clones were injected into C57BL/6 blastocysts. Mice were crossed to Flp-expressing mice to delete the neo cassette. (J:238627)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Ca1 (mouse) Summary of all recombinase alleles driven by Ca1. Alliance mouse Ca1 gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ca1 Mutation: 12 strains or lines available

References

• Original: J:238627 Tetteh PW, et al., Generation of an inducible colon-specific Cre enzyme mouse line for colon cancer research. Proc Natl Acad Sci U S A. 2016 Oct 18;113(42):11859-11864
• All: 1 reference(s)