Mcm3^tm1.1Geno
Targeted Allele Detail

Summary

• Symbol: Mcm3^tm1.1Geno
• Name: minichromosome maintenance complex component 3; targeted mutation 1.1, Genoway
• MGI ID: MGI:5907491
• Synonyms: Mcm3^Lox
• Gene: Mcm3 Location: Chr1:20873192-20890536 bp, - strand Genetic Position: Chr1, 6.5 cM, cytoband A3-A5
• Alliance: Mcm3^tm1.1Geno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:227638
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Conditional ready, Hypomorph, Reporter)
• Mutation: Insertion
• Mutation details: A loxP site was inserted in intron 13 and IRES-EGFP-Luciferase cassette, FRT flanked neo cassette and loxP site were inserted after the stop codon in exon 17. Flp mediated recombination removed the neo cassette. In heterozygous and homozygous MEFs, mRNA expression is reduced to about 70% and 30% of wild-type levels. (J:227638)

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mcm3 Mutation: 43 strains or lines available

References

• Original: J:227638 Alvarez S, et al., Replication stress caused by low MCM expression limits fetal erythropoiesis and hematopoietic stem cell functionality. Nat Commun. 2015;6:8548
• All: 1 reference(s)