Atoh1^{tm1(Neurog1)Bfri}
Targeted Allele Detail

Summary

• Symbol: Atoh1^{tm1(Neurog1)Bfri}
• Name: atonal bHLH transcription factor 1; targeted mutation 1, Bernd Fritzsch
• MGI ID: MGI:5907327
• Synonyms: Atoh1^KINeurog1, Atoh1^tm1Bfri
• Gene: Atoh1 Location: Chr6:64706109-64708229 bp, + strand Genetic Position: Chr6, 30.03 cM
• Alliance: Atoh1^{tm1(Neurog1)Bfri} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:242970
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Epitope tag, Inserted expressed sequence, Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: In this knock-in mutation, the coding region of the endogenous gene except for the first 15 base pairs has been replaced by a full-length cDNA encoding mouse neurogenin 1 with an amino-terminal 6X Myc epitope tag, an internal ribosomal entry site (IRES) followed by the coding sequence for the DsRed2 fluorescent protein, and a loxP site-flanked PGK-neo cassette. In homozygous mutant embryos, no Atoh1 expression is detected by in situ hybridization at embryonic day (E) 14.5 or E18.5. Heterozygous embryos exhibit reduced cochlear expression of Atoh1 relative to wild-type littermates. In E14.5 homozygous mutants, Atoh1 promoter-driven Neurog1 expression is observed in vestibular sensory epithelia and the cochlear mid-base; it is expanded in delaminating neuroblasts, as in Atoh1 knock-outs. By E18.5 Neurog1 is detected only in clusters of organ of Corti cells except for continuous expression at the apical tip. Heterozygotes at E14.5 express Neurog1 ectopicaly in vestibular hair cells, while endogenous Neurog1 expression is reduced in delaminating neuroblasts. Atoh1 and Neurog1 are co-expressed in hair cells in E18.5 heterozygotes. (J:242970)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Atoh1 Mutation: 36 strains or lines available

References

• Original: J:242970 Jahan I, et al., Expression of Neurog1 instead of Atoh1 can partially rescue organ of Corti cell survival. PLoS One. 2012;7(1):e30853
• All: 3 reference(s)