Prkag2^tm1.1Geno
Targeted Allele Detail

Summary

• Symbol: Prkag2^tm1.1Geno
• Name: protein kinase, AMP-activated, gamma 2 non-catalytic subunit; targeted mutation 1.1, Genoway
• MGI ID: MGI:5907189
• Synonyms: R299Q gamma2 AMPK
• Gene: Prkag2 Location: Chr5:25067742-25305640 bp, - strand Genetic Position: Chr5, 11.93 cM
• Alliance: Prkag2^tm1.1Geno page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:235379
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129S/SvEv

Mutation description

• Allele Type: Targeted (Conditional ready, Constitutively active)
• Mutations: Insertion, Single point mutation
• Mutation details: A c.896G>A mutation was engineered in exon 7 changing arginine codon 299 to a glutamine codon (p.Arg299Gln). This mutation is the equivalent of the human R302Q mutation which causes increased unstimulated activity and is associated with a cardiac phenotype. An FRT site flanked neomycin resistance gene cassette was inserted into intron 6 and removed later through flp-mediated recombination. In addition, loxP sites were inserted within intron 6 upstream and within intron 7 downstream from the mutation. (J:235379, J:256296)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Prkag2 Mutation: 55 strains or lines available

References

• Original: J:235379 Yavari A, et al., Chronic Activation of gamma2 AMPK Induces Obesity and Reduces beta Cell Function. Cell Metab. 2016 May 10;23(5):821-36
• All: 4 reference(s)