Gt(ROSA)26Sor^{tm3(CAG-Cpt1a*)Ics}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm3(CAG-Cpt1a*)Ics}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 3, Mouse Clinical Institute
• MGI ID: MGI:5907179
• Synonyms: LoxP-CPT1mt^tg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm3(CAG-Cpt1a*)Ics} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241405
• Parent Cell Line: S3 (ES Cell)
• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The following elements were inserted into the locus: a loxP site flanked STOP cassette (consisting of a neomycin resistance gene cassette followed by three SV40 poly(A) signal sequences), the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin (CAG) hybrid promoter, and the rat gene with a p.Met593Ser mutation. This creates a conditional-ready allele from which the rat gene will only be expressed after cre-mediated recombination to remove the STOP cassette. (J:241405)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1089 strains or lines available

References

• Original: J:241405 Vavrova E, et al., Muscle expression of a malonyl-CoA-insensitive carnitine palmitoyltransferase-1 protects mice against high-fat/high-sucrose diet-induced insulin resistance. Am J Physiol Endocrinol Metab. 2016 Sep 01;311(3):E649-60
• All: 2 reference(s)