Agtr1a^{tm1.1(cre)Ekrs}
Targeted Allele Detail

Summary

• Symbol: Agtr1a^{tm1.1(cre)Ekrs}
• Name: angiotensin II receptor, type 1a; targeted mutation 1.1, Eric Krause
• MGI ID: MGI:5906996
• Synonyms: AT1aR-Cre
• Gene: Agtr1a Location: Chr13:30520424-30566850 bp, + strand Genetic Position: Chr13, 13.19 cM
• Alliance: Agtr1a^{tm1.1(cre)Ekrs} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:240544
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Recombinase, Reporter)
• Mutation: Insertion
• Agtr1a^{tm1.1(cre)Ekrs} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Agtr1a (MGI:87964)

• Mutation details: A targeting construct was designed to insert a Cre Recombinase gene and a zsGreen (enhanced green fluorescence protein) just upstream of the 3'UTR of the angiotensin II receptor, type 1a (Agtr1a) gene. Viral P2A oligopeptides, from porcine teschovirus-1, were inserted upstream and downstream of the cre gene to enable ribosomal skipping. A frt-flanked neomycin resistance (neo) cassette was placed downstream of the 3'UTR. This construct was electroporated into C57BL/6J embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and the resulting chimeric mice were bred to FLP (FLPo) recombinase expressing mice to delete the neo cassette. (J:240544)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Agtr1a (mouse) Summary of all recombinase alleles driven by Agtr1a. Comparative matrix view of recombinase activities Alliance mouse Agtr1a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Agtr1a Mutation: 40 strains or lines available

References

• Original: J:240544 de Kloet AD, et al., A Unique "Angiotensin-Sensitive" Neuronal Population Coordinates Neuroendocrine, Cardiovascular, and Behavioral Responses to Stress. J Neurosci. 2017 Mar 29;37(13):3478-3490
• All: 4 reference(s)