H2al1m^tm1Skho
Targeted Allele Detail

Summary

• Symbol: H2al1m^tm1Skho
• Name: H2A histone family member L1M; targeted mutation 1, Saadi Khochbin
• MGI ID: MGI:5906924
• Synonyms: h2a.l.2^Neo
• Gene: H2al1m Location: ChrX:9150003-9150527 bp, + strand Genetic Position: ChrX, 4.22 cM, cytoband A1.2
• Alliance: H2al1m^tm1Skho page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241000
• Parent Cell Line: AT1 (ES Cell)
• Strain of Origin: 129S2/SvPasCrl

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout)
• Mutation: Insertion
• Mutation details: A TAP (6His-Flag-Ha) tag was inserted in frame with the last exon. A loxP site flanked neomycin resistance gene cassette was inserted downstream of the tag. The neo cassette interferes with the expression of the gene, creating a null allele. (J:241000)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any H2al1m Mutation: 0 strains or lines available

References

• Original: J:241000 Barral S, et al., Histone Variant H2A.L.2 Guides Transition Protein-Dependent Protamine Assembly in Male Germ Cells. Mol Cell. 2017 Apr 06;66(1):89-101.e8
• All: 1 reference(s)