Idh2^tm1Meln
Targeted Allele Detail

Summary

• Symbol: Idh2^tm1Meln
• Name: isocitrate dehydrogenase 2 (NADP+), mitochondrial; targeted mutation 1, Ari Melnick
• MGI ID: MGI:5906615
• Synonyms: Idh2^R140Q
• Gene: Idh2 Location: Chr7:79744594-79765140 bp, - strand Genetic Position: Chr7, 45.43 cM
• Alliance: Idh2^tm1Meln page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241017
• Parent Cell Line: BAC-BA1 (ES Cell)
• Strain of Origin: (C57BL/6 x 129S/SvEv)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Null/knockout)
• Mutations: Insertion, Single point mutation
• Mutation details: A c.419G>A mutation was engineered in exon 4, changing arginine codon 140 into a glutamine codon (p.Arg140Gln). This mutation is found in tumors from human accute myeloid leukemia (AML) patients. A loxP site flanked neomycin resistance gene and stop cassette was inserted into intron 3. This creates a conditional-ready null allele that expresses the mutated protein only after cre-mediated recombination. (J:241017)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Idh2 Mutation: 145 strains or lines available

References

• Original: J:241017 Shih AH, et al., Combination Targeted Therapy to Disrupt Aberrant Oncogenic Signaling and Reverse Epigenetic Dysfunction in IDH2- and TET2-Mutant Acute Myeloid Leukemia. Cancer Discov. 2017 May;7(5):494-505
• All: 4 reference(s)