Tpk1^em1(IMPC)J
Endonuclease-mediated Allele Detail

Summary

• Symbol: Tpk1^em1(IMPC)J
• Name: thiamine pyrophosphokinase; endonuclease-mediated mutation 1, Jackson
• MGI ID: MGI:5905734
• Synonyms: Tpk1^-
• Gene: Tpk1 Location: Chr6:43321935-43643212 bp, - strand Genetic Position: Chr6, 21.19 cM, cytoband B2
• Alliance: Tpk1^em1(IMPC)J page
• IMPC: Tpk1 gene page

Tpk1^em1(IMPC)J/Tpk1^em1(IMPC)J mice exhibit embryonic lethality, with embryos recovered at E7.5 but not at E9.5. Embryos are smaller and form a rudimentary egg cylinder but no primitive streak or hallmarks of gastrulation are seen at E7.5.

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Strain of Origin: C57BL/6NJ
• Project Collection: IMPC

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: This allele was generated at The Jackson Laboratory using Cas9 and guides with spacer sequences CCAGCGAGTCAGGGTCTACA, CCAGCTTCGGTAATAAAAGA, TAGGAAACCACCTCTGTGAG, and TCTCCAGTCCATCTGAACAG that targeted exon(s) ENSMUSE00000258146.4. This resulted in deletion(s) of 2 bp (location: Chr6:43537352-43537353; GRCm39), 4 bp (location: Chr6:43537216-43537219; GRCm39), and 415 bp (location: Chr6:43536752-43537166; GRCm39). This description was generated automatically. Additional molecular information including FASTA sequence support for this allele can be found here: (IMPC Gene page) and viewed in a genome context here: (IMPC Genome Browser). (J:384794)

• Inheritance: Not Specified

Expression

In Structures Affected by this Mutation:

3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Tpk1 Mutation: 24 strains or lines available

References

• Original: J:188991 The Jackson Laboratory, Alleles produced for the KOMP project by The Jackson Laboratory. MGI Direct Data Submission. 2012
• All: 5 reference(s)