Htr1a^{tm1.1(cre)Hze}
Targeted Allele Detail

Summary

• Symbol: Htr1a^{tm1.1(cre)Hze}
• Name: 5-hydroxytryptamine (serotonin) receptor 1A; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5904051
• Synonyms: Htr1a-IRES2-Cre-D
• Gene: Htr1a Location: Chr13:105580147-105584630 bp, + strand Genetic Position: Chr13, 56.92 cM
• Alliance: Htr1a^{tm1.1(cre)Hze} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:146821
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Recombinase)
• Mutation: Insertion
• Htr1a^{tm1.1(cre)Hze} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Htr1a (MGI:96273)

• Mutation details: The targeting vector contained, from 5' to 3', a partial Htr1a sequence spanning exon 1 (including the endogenous stop codon), an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. This construct was electroporated into (129S6/SvEvTac x C57BL/6)F1-derived G4 embryonic stem (ES) cells. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). Cre recombinase expression/activity is observed mainly in cortex, hippocampus and retro-hippocamal regions, as well as scattered neurons elsewhere in the brain. (J:146821)

Recombinase activity

Activity:	Tissue activity of this recombinase allele
Driver:	Htr1a (mouse) Summary of all recombinase alleles driven by Htr1a. Alliance mouse Htr1a gene page

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Htr1a Mutation: 26 strains or lines available

References

• Original: J:146821 Allen Institute for Brain Science, Cre transgenes and knockin alleles created at the Allen Insitute for Brain Science (AIBS). MGI Direct Data Submission. 2009-04-06
• All: 4 reference(s)