Chrm2^tm1.1Hze
Targeted Allele Detail

Summary

• Symbol: Chrm2^tm1.1Hze
• Name: cholinergic receptor, muscarinic 2, cardiac; targeted mutation 1.1, Hongkui Zeng
• MGI ID: MGI:5904011
• Synonyms: Chrm2-tdT-D
• Gene: Chrm2 Location: Chr6:36365019-36505349 bp, + strand Genetic Position: Chr6, 15.48 cM
• Alliance: Chrm2^tm1.1Hze page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector contains, from 5' to 3', a partial Chrm2 sequence spanning exon 4 (not including the endogenous stop codon), a polylinker sequence, a tdTomato sequence, a WPRE sequence, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::frt5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Chrm2 Mutation: 32 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 6 reference(s)