Igdmr^{em1(Snrpn-EGFP)Jae}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Igdmr^{em1(Snrpn-EGFP)Jae}
• Name: intergenic germline-derived differentially methylated region; endonuclease-mediated mutation 1, Rudolf Jaenisch
• MGI ID: MGI:5903915
• Synonyms: IG-DMR^GFP
• Gene: Igdmr Location: unknown Genetic Position: Chr12, Syntenic
• Alliance: Igdmr^{em1(Snrpn-EGFP)Jae} page

Mutation origin

• Strain of Origin: (129 x CAST)F1

Mutation description

• Allele Type: Endonuclease-mediated (Reporter)
• Mutation: Insertion
• Mutation details: CRISPR-Cas9 methodology is used to introduce a knock-in DNA methylation reporter (Snrpn*/GFP) adjacent to the 3' end of the Dlk1-Dio3 locus on mouse chromosome 12 (the IG-DMR, intergenic differentially methylated region).The Snrpn*/GFP cassette contains a GFP fused to the minimal promoter region of the mouse Snrpn gene; it includes conserved elements and the endogenous imprinted differentially methylated region. Maternal inheritance of the reporter allele is required to express the GFP reporter. (J:239124)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Igdmr Mutation: 2 strains or lines available

Notes

ES cells = F123

References

• Original: J:239124 Stelzer Y, et al., Parent-of-Origin DNA Methylation Dynamics during Mouse Development. Cell Rep. 2016 Sep 20;16(12):3167-80
• All: 3 reference(s)