Dot1l^tm1Wcs
Targeted Allele Detail

Summary

• Symbol: Dot1l^tm1Wcs
• Name: DOT1 like histone lysine methyltransferase; targeted mutation 1, William C Skarnes
• MGI ID: MGI:5903511
• Synonyms: Dot1l^tm2
• Gene: Dot1l Location: Chr10:80591040-80631295 bp, + strand Genetic Position: Chr10, 39.72 cM
• Alliance: Dot1l^tm1Wcs page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243553
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Duplication, Insertion
• Mutation details: A splice acceptor, hygromycin resistance gene, GFP reporter gene, polyadenylation sequence and kanamycin resistance gene were inserted upstream of the critical exon 2, duplicating genomic sequences contained in the vector. (J:243553)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Dot1l Mutation: 75 strains or lines available

References

• Original: J:243553 Fisher CL, et al., An efficient method for generation of bi-allelic null mutant mouse embryonic stem cells and its application for investigating epigenetic modifiers. Nucleic Acids Res. 2017 Dec 1;45(21):e174
• All: 1 reference(s)