Fgfr1^tm11.1Sor
Targeted Allele Detail

Summary

• Symbol: Fgfr1^tm11.1Sor
• Name: fibroblast growth factor receptor 1; targeted mutation 11.1, Philippe Soriano
• MGI ID: MGI:5903015
• Synonyms: Fgfr1-Cerulean, Fgfr1-T2A-H2B-Cerulean
• Gene: Fgfr1 Location: Chr8:26008808-26067819 bp, + strand Genetic Position: Chr8, 14.12 cM
• Alliance: Fgfr1^tm11.1Sor page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:241560
• Parent Cell Line: AK7.1 (ES Cell)
• Strain of Origin: 129S4/SvJaeSor

Mutation description

• Allele Type: Targeted (No functional change, Reporter)
• Mutation: Insertion
• Mutation details: The targeting vector is designed to replace the stop codon in exon 18 of the Fgfr1 gene with a cassette containing a human HIST1H2BB gene [histone 1 H2bb] sequence, a viral 2A oligopeptide (T2A) that mediates ribosomal skipping, a cerulean variant of green fluorescent protein (GFP) sequence, and a new stop codon. An FRT-flanked neomycin resistance (neo) cassette was inserted downstream of the new stop codon. Flp-mediated recombination removed the FRT-flanked neo cassette. (J:241560)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Fgfr1 Mutation: 221 strains or lines available

References

• Original: J:241560 Molotkov A, et al., Distinct Requirements for FGFR1 and FGFR2 in Primitive Endoderm Development and Exit from Pluripotency. Dev Cell. 2017 Jun 05;41(5):511-526.e4
• All: 1 reference(s)